Loading bufer with the fluorescent dye SYBR^® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.

ROTI^®Load DNAstain SYBR^® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR^® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR^® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.SYBR^® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE^®PROfessional runVIEW, Art. No. 4849.1).When excited, nucleic acid-bound SYBR^® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR^® Green foto filters.The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI^®Load DNAstain SYBR^® Green.

Excitation maximum (bound to DNA): 254 nm and 495 nmEmission maximum (bound to DNA): 521 nm

ROTI^®Load DNAstain 3 Greenis a special gel loading buffer with orange G as the only tracking dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs. Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands.

Glycerin is the standard reagent for increasing sample density.

Recommended for staining of dsDNA- and dsRNA-fragments of under 500 bp.