In thin-layer chromatography, the separation of the components of a mixture is the most delicate step, as it is subject to different and variable parameters.

Some are perfectly controlled (chemical nature of the stationary and mobile phase), others are more random (degree of humidity, ambient temperature, pre-saturation).

All require precise and rigorous protocols to achieve correct, repeatable and reproducible separations. The quality of the migration separation chamber and the choice of its size and shape are very important.

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