SUFU Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8416-50, BS8416-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UMX1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P IF/ICC

All Applications: WB: 1:500~1:2000
IHC-P/IF/ICC: 1:50~1:200

Background: Su(fu) (for Suppressor-of-Fused) is a key negative regulator in the vertebrate Hedgehog signaling pathway. Su(fu) interacts with genes encoding proteins in this signal transduction pathway. In Drosophila, Intracellular transduction of the Hedgehog pathway involves the release of a large complex containing Su(fu). Su(fu) inhibits the activity of the transcription factor Gli1 and interacts with Gli2, Gli3 and the serine/threonine kinase Fused. Su(fu) is widely expressed in adult and embryonic tissues with higher expression in tissues patterned by hegdehog signaling. The Su(fu) gene locus maps to a region that is deleted in glioblastomas, prostate cancer, malignant melanoma and endometrial cancer.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SUFU polyclonal antibody detects endogenous levels of SUFU protein.

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using SUFU pAb at 1:1000 dilution., Immunohistochemistry analysis of paraffin-embedded human breast cancer using SUFU pAb at dilution of 1:100 (40x lens)., Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SUFU pAb at dilution of 1:100 (40x lens)., Immunofluorescence analysis of MCF-7 cells using SUFU antibody.

Alternative Name: Suppressor of fused homolog; SUFUH; SUFU; UNQ650; PRO1280

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human SUFU (NP_057253.2).

Conjugate: Unconjugated

Modification: Unmodified