PRKX Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS5865-50, BS5865-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51817

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. PRKY (protein kinase, Y-linked) and PRKX (protein kinase, X-linked) are members of the Ser/Thr protein kinase family, both of which belong to the subfamily of cAMP-dependent kinases. Encoded by a gene that is located near the pseudoautosomal region on chromosome Y, PRKY contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins. Functioning in a similar manner to PRXY, PRKX contains one protein kinase domain and is essential for macrophage differentiation and renal epithelial cell migration. Defects in the genes encoding PRKY and PRXX are associated with sex reversal disorder, namely XX in males and XY in females.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PRKX polyclonal antibody detects endogenous levels of PRKX protein.

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PRKX polyclonal antibody at 1:500 dilution
Lane 1: HEK29T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate, Immunohistochemistry (IHC) analyzes of PRKX pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of PRKX pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of PRKX pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: cAMP-dependent protein kinase catalytic subunit PRKX; PrKX; Protein kinase X; Protein kinase X-linked; Serine/threonine-protein kinase PRKX; Protein kinase PKX1; PKX1

Immunogen: Synthetic peptide, corresponding to amino acids 308-353 of Human PRKX.

Conjugate: Unconjugated

Modification: Unmodified