BRD0539 is a potent inhibitor of Streptococcus pyogenes Cas9 (SpCas9) with IC50 of 22 μM in an in vitro DNA cleavage assay.

Product information

CAS Number: 1403838-79-8

Molecular Weight: 452.54

Formula: C25H25FN2O3S

Chemical Name: (3aR, 4R, 9bR)-8-(2-Fluorophenyl)-2, 3, 3a, 4, 5, 9b-hexahydro-1-[(4-methylphenyl)sulfonyl]-1H-pyrrolo[3, 2-c]quinoline-4-methanol

Smiles: CC1C=CC(=CC=1)S(=O)(=O)N1CC[C@@H]2[C@H](CO)NC3=CC=C(C=C3[C@H]12)C1=CC=CC=C1F

InChiKey: CZOIXFMISSBIJL-DCEDVJGZSA-N

InChi: InChI=1S/C25H25FN2O3S/c1-16-6-9-18(10-7-16)32(30,31)28-13-12-20-24(15-29)27-23-11-8-17(14-21(23)25(20)28)19-4-2-3-5-22(19)26/h2-11,14,20,24-25,27,29H,12-13,15H2,1H3/t20-,24+,25-/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO: 91 mg/mL(201.08 mM).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

BRD0539 is stable in human plasma and reversibly inhibits SpCas9. BRD0539 does not interfere with the SpCas9:gRNA interaction. BRD0539 dose-dependently blocks the formation of the DNA-bound state in a dose-dependent fashion. BRD0539 impairs the perturbation induced by the 4PAM DNA. BRD0539 is able to inhibit SpCas9 in the eGFP-disruption assay.

References:

1. Basudeb Maji, et al. Cell. 2019 May 2;177(4):1067-1079.e19.

Products are for research use only. Not for human use.