PSMD12 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65319-50, BS65319-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: O00232

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Background: proteasome 26S subunit, non-ATPase 12(PSMD12) Homo sapiens The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PSMD12 Polyclonal Antibody detects endogenous levels of PSMD12 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of various cells using PSMD12 Polyclonal Antibody, Western Blot analysis of HepG2 cells using PSMD12 Polyclonal Antibody, Western blot analysis of lysates from HepG2 cells, using PSMD12 Antibody. The Lane on the right is blocked with the synthesized peptide., Western blot analysis of the lysates from HT-29 cells using PSMD12 antibody.

Alternative Name: PSMD12; 26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit RPN5; 26S proteasome regulatory subunit p55

Immunogen: The antiserum was produced against synthesized peptide derived from human PSMD12. AA range:151-200

Conjugate: Unconjugated

Modification: Unmodified