RhoA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16454-50, BZ16454-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P61586

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, WB, IHC-F, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Rho A is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RhoA in HepG2 lysates using RhoA antibody., Immunofluorescence analysis of RhoA in rat lung using Rho A antibody, and DAPI., Immunofluorescence analysis of RhoA in mouse lung using Rho A antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human stomach cancer tissue using Rho A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Negative control was used by secondary antibody only.

Alternative Name: RHOA; ARH12; ARHA; RHO12; Transforming protein RhoA; Rho cDNA clone 12; h12

Immunogen: The antiserum was produced against synthesized peptide derived from human RhoA. AA range:144-193

Conjugate: Unconjugated

Modification: Unmodified