PKC zeta Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16387-50, BZ16387-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q05513

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: PKC is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 68 kDa; Observed MW: 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PKC zeta in various lysates using PKC zeta antibody., Western blot analysis of PKC zeta in COS7 lysates using PKC ζ antibody., Immunofluorescence analysis of PKC zeta in.NIH/3T3 cells, using PKC zeta antibody. The picture on the right is blocked with the synthesized peptide., Immunohistochemistry analysis of paraffin-embedded Human brain tissue using PKC zeta antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Alternative Name: PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC zeta. AA range:526-575

Conjugate: Unconjugated

Modification: Unmodified