eNOS Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16451-50, BZ16451-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P29474

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 133 kDa; Observed MW: 140 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of eNOS in HepG2 lysates using eNOS antibody., Western blot analysis of eNOS in HepG2 lysates using NOS3 antibody., Immunohistochemistry analysis of paraffin-embedded Human brain using eNOS antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Sample with blocking peptide on the right., Western blot analysis of eNOS in Jurkat lysates treated with Insulin using eNOS antibody. The Lane on the right is blocked with the synthesized peptide.

Alternative Name: NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

Immunogen: The antiserum was produced against synthesized peptide derived from human eNOS. AA range:1144-1193

Conjugate: Unconjugated

Modification: Unmodified